| Ct Reference gene | Ct Gene of interest | |
| Control#1 | ||
| Control#2 | ||
| Control#3 | ||
| Treatment#1 | ||
| Treatment#2 | ||
| Treatment#3 |
| delta Ct | delta delta Ct | Relative expression | |
| Control#1 | |||
| Control#2 | |||
| Control#3 | |||
| Treatment#1 | |||
| Treatment#2 | |||
| Treatment#3 |
| Mean Expression Ratio | |
| Control | |
| Treatment |
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